Genetic analyses have shaped much of our understanding of cancer. However, it is becoming
increasingly clear that cancer cells display features of normal tissue organization, where …

Intratumoral heterogeneity arises through the evolution of genetically diverse subclones
during tumor progression. However, it remains unknown whether cells within single genetic …

The cancer stem cell (CSC) or cancer-initiating cancer (C-IC) model has garnered considerable
attention over the past several years since Dick and colleagues published a seminal …

Tumor recurrence following treatment remains a major clinical challenge. Evidence from
xenograft models and human trials indicates selective enrichment of cancer-initiating cells (CICs…

The blood system is sustained by a pool of haematopoietic stem cells (HSCs) that are long-lived
due to their capacity for self-renewal. A consequence of longevity is exposure to stress …

There is increasing evidence that some cancers are hierarchically organized, sustained by
a relatively rare population of cancer-initiating cells (C-ICs). Although the capacity to initiate …

It has long been appreciated that significant functional and morphologic heterogeneity can
exist within the individual cells that comprise a tumor. Increasing evidence indicates that …

The hematopoietic system sustains regeneration throughout life by balancing self-renewal
and differentiation. To stay poised for mature blood production, hematopoietic stem cells (…

Lifelong maintenance of the blood system requires equilibrium between clearance of
damaged hematopoietic stem cells (HSCs) and long-term survival of the HSC pool. Severe …

This unit describes protocols for working with colon cancer stem cells. To work with these
cells one must start by generating single‐cell suspensions from human colon cancer tissue. …